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Article
UniFrag and GenomePrimer, programs to select primers for genome-wide production of unique amplicons
Sacha A.F.T. van Hijum, Anne de Jong*, Girbe Buist, Jan Kok, and Oscar P. Kuipers
Abstract
The complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility